pCAG2IG
(Plasmid
#122292)
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Purpose(Empty Backbone) Overexpression control
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122292 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAGIG
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Backbone manufacturerConnie Cepko, Addgene plasmid #11159
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Modifications to backboneBamH1 restriction site is added into pCAGIG MCS.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Addgene NGS unable to fully resolve CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG2IG was a gift from Yukiko Gotoh (Addgene plasmid # 122292 ; http://n2t.net/addgene:122292 ; RRID:Addgene_122292) -
For your References section:
HMGA regulates the global chromatin state and neurogenic potential in neocortical precursor cells. Kishi Y, Fujii Y, Hirabayashi Y, Gotoh Y. Nat Neurosci. 2012 Aug;15(8):1127-33. doi: 10.1038/nn.3165. 10.1038/nn.3165 PubMed 22797695
