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Addgene

pHMGWA-Cas1
(Plasmid #122285)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122285 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHMGWA
  • Backbone size w/o insert (bp) 6626
  • Total vector size (bp) 7544

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Eco Cas1
  • Insert Size (bp)
    918

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHMGWA-Cas1 was a gift from Jennifer Doudna (Addgene plasmid # 122285 ; http://n2t.net/addgene:122285 ; RRID:Addgene_122285)

  • For your References section:

    Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nunez JK, Kranzusch PJ, Noeske J, Wright AV, Davies CW, Doudna JA. Nat Struct Mol Biol. 2014 Jun;21(6):528-34. doi: 10.1038/nsmb.2820. Epub 2014 May 4. 10.1038/nsmb.2820 PubMed 24793649
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