BSP605
(Plasmid
#122250)
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Purposeexpression vector for mCerulean under EFT3 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122250 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBSP188
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Vector typeWorm Expression
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Selectable markersG418 in yeast
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCerulean
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SpeciesC. elegans (nematode)
- Promoter EFT3
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CGTAATACGACTCACTtaAGGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BSP605 was a gift from Alexander Mendenhall (Addgene plasmid # 122250 ; http://n2t.net/addgene:122250 ; RRID:Addgene_122250) -
For your References section:
A toolkit for DNA assembly, genome engineering and multicolor imaging for C. elegans. Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A. Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. Epub 2018 Apr 13. 10.1016/j.tma.2018.01.001 PubMed 33693300
