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Addgene

pLKO-PHB-8
(Plasmid #122233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA targeting PHB (see partial depositor seq)
  • Alt name
    shRNA TRCN0000029208
  • Alt name
    shPHB8
  • gRNA/shRNA sequence
    CDS
  • Species
    H. sapiens (human)
  • Entrez Gene
    PHB1 (a.k.a. HEL-215, HEL-S-54e, PHB)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LKO.1 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO-PHB-8 was a gift from Alex Kentsis (Addgene plasmid # 122233 ; http://n2t.net/addgene:122233 ; RRID:Addgene_122233)

  • For your References section:

    Prohibitin is a prognostic marker and therapeutic target to block chemotherapy resistance in Wilms' tumor. Ortiz MV, Ahmed S, Burns M, Henssen AG, Hollmann TJ, MacArthur I, Gunasekera S, Gaewsky L, Bradwin G, Ryan J, Letai A, He Y, Naranjo A, Chi YY, LaQuaglia M, Heaton T, Cifani P, Dome JS, Gadd S, Perlman E, Mullen E, Steen H, Kentsis A. JCI Insight. 2019 Aug 8;4(15). pii: 127098. doi: 10.1172/jci.insight.127098. eCollection 2019 Aug 8. 10.1172/jci.insight.127098 PubMed 31391345
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