plx304-PHB-V5
(Plasmid #122230)

• Purpose: Expresses human PHB attached to a V5 tag
• Depositing Lab: Alex Kentsis
• Publication: Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 127098. doi: 10.1172/jci.insight.127098. eCollection 2019 Aug 8.

Backbone

• Vector backbone: pLX304
• Backbone size w/o insert (bp): 7700
• Total vector size (bp): 8600
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Prohibitin
• Alt name: PHB
• Species: H. sapiens (human)
• Insert Size (bp): 951
• GenBank ID: NM_001281496.1
• Entrez Gene: PHB1 (a.k.a. HEL-215, HEL-S-54e, PHB)
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry