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Rescue myc-PCDHGC5 mRNA
(Plasmid #122229)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122229 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA 3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCDHGC5
  • Species
    R. norvegicus (rat)
  • Mutation
    Silent mutations are in five consecutive codons (ACATCTGAAGCAGTG to ACGTCCGAGGCTGTTboth encoding TSEAV) in the PCDHGC5 sh1 shRNA target site
  • Entrez Gene
    Pcdhgc5
  • Tag / Fusion Protein
    • 9E10 cMyc epitope (EQKLISEEDL) was inserted between amino acids 103 and 104 of mature protein (after signal peptide cleavage) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert was originally cloned in Angel de Blas's lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rescue myc-PCDHGC5 mRNA was a gift from Angel de Blas (Addgene plasmid # 122229 ; http://n2t.net/addgene:122229 ; RRID:Addgene_122229)

  • For your References section:

    Molecular and functional interaction between protocadherin-gammaC5 and GABAA receptors. Li Y, Xiao H, Chiou TT, Jin H, Bonhomme B, Miralles CP, Pinal N, Ali R, Chen WV, Maniatis T, De Blas AL. J Neurosci. 2012 Aug 22;32(34):11780-97. doi: 10.1523/JNEUROSCI.0969-12.2012. 10.1523/JNEUROSCI.0969-12.2012 PubMed 22915120
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