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Addgene

PCDHGC5 sh1 shRNA
(Plasmid #122227)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122227 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mU6pro
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCDHGC5 shRNA
  • gRNA/shRNA sequence
    UCUUCACUGCUUCAGAUGUAU
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Pcdhgc5

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert was originally cloned in Angel de Blas's lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCDHGC5 sh1 shRNA was a gift from Angel de Blas (Addgene plasmid # 122227 ; http://n2t.net/addgene:122227 ; RRID:Addgene_122227)

  • For your References section:

    Molecular and functional interaction between protocadherin-gammaC5 and GABAA receptors. Li Y, Xiao H, Chiou TT, Jin H, Bonhomme B, Miralles CP, Pinal N, Ali R, Chen WV, Maniatis T, De Blas AL. J Neurosci. 2012 Aug 22;32(34):11780-97. doi: 10.1523/JNEUROSCI.0969-12.2012. 10.1523/JNEUROSCI.0969-12.2012 PubMed 22915120
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