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Addgene

Foxf1 pGEX4T1
(Plasmid #122213)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122213 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Foxf1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1062
  • Entrez Gene
    Foxf1 (a.k.a. FREAC1, Foxf1a, Freac-1, HFH-8, Hfh8)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pGEX 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Foxf1 pGEX4T1 was a gift from Paul Herring (Addgene plasmid # 122213 ; http://n2t.net/addgene:122213 ; RRID:Addgene_122213)

  • For your References section:

    The transcription factor Foxf1 binds to serum response factor and myocardin to regulate gene transcription in visceral smooth muscle cells. Hoggatt AM, Kim JR, Ustiyan V, Ren X, Kalin TV, Kalinichenko VV, Herring BP. J Biol Chem. 2013 Oct 4;288(40):28477-87. doi: 10.1074/jbc.M113.478974. Epub 2013 Aug 14. 10.1074/jbc.M113.478974 PubMed 23946491
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