pcDNA3.1-myc-BirA*-NRASG12V
(Plasmid #122209)

• Purpose: Expresses myc-BirA* fused to NRASG12V in mammalian cells
• Depositing Lab: Christopher Counter
• Publication: Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 10.1038/s41467-018-05692-6.

Backbone

• Vector backbone: pcDNA3.1 myc BioID
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 5967
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NRASG12v
• Alt name: NRAS
• Species: H. sapiens (human)
• Insert Size (bp): 567
• Mutation: changed G12 to V
• GenBank ID: 4893
• Entrez Gene: NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc-BirA* (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry