Skip to main content
Addgene

lenti_dCas9-KRAB-MeCP2
(Plasmid #122205)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMH0001
  • Modifications to backbone
    Added MeCP2 fusion to dCas9-KRAB and swapped the selection marker from BFP to BSD.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-KRAB-MeCP2
  • Alt name
    dCas9-KM
  • Species
    Synthetic
  • Insert Size (bp)
    5300
  • Mutation
    Cas9 (D10A, H840A)
  • Tag / Fusion Protein
    • KRAB-MeCP2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SbfI (unknown if destroyed)
  • 5′ sequencing primer AACGGCAGCAGCGGATC
  • 3′ sequencing primer CGACCTGCAGGCATGCAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti_dCas9-KRAB-MeCP2 was a gift from Andrea Califano (Addgene plasmid # 122205 ; http://n2t.net/addgene:122205 ; RRID:Addgene_122205)

  • For your References section:

    Interrogation of genome-wide, experimentally dissected gene regulatory networks reveals mechanisms underlying dynamic cellular state control. Tan X, Worley J, Turunen M, Wong K, Fernández EC, Paull E, Jones S, Wang J, Noh H, Salvatori B, Chavez A, Califano A. bioRxiv 2021.06.28.449297 10.1101/2021.06.28.449297
Related items:
Commonly requested with: