LentiV_Neo_POU2F3_CR
(Plasmid #122175)

• Purpose: Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene
• Depositing Lab: Christopher Vakoc
• Publication: Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928. doi: 10.1101/gad.314815.118. Epub 2018 Jun 26.

Backbone

• Vector backbone: LentiV_Neo
• Backbone size w/o insert (bp): 7183
• Total vector size (bp): 8491
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: POU2F3
• Alt name: OCT11
• Species: H. sapiens (human)
• Insert Size (bp): 1308
• Mutation: 3 silent mutations for gRNA resistance (882g-c, 885t-c, 888g-c)
• GenBank ID: NM_014352.4 NM_014352.4
• Entrez Gene: POU2F3 (a.k.a. Epoc-1, OCT-11, OCT11, OTF-11, PLA-1, PLA1, Skn-1a)
• Promoter: EFS

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CCGCCACCatggtgaatctggagtccatg
• 3′ sequencing primer: CCGCTGCCgtggaggtaggtggaatgattc

Resource Information

• A portion of this plasmid was derived from a plasmid made by: DNASU

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry