pET His6 MBP TEV HDAC8_374
(Plasmid #122174)

• Purpose: Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
• Depositing Lab: David Christianson
• Publication: Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-2286. doi: 10.1021/acschembio.7b00330. Epub 2017 Aug 30.

Backbone

• Vector backbone: pET His6 MBP TEV LIC cloning vector
• Backbone manufacturer: Scott Gradia
• Backbone size w/o insert (bp): 6465
• Total vector size (bp): 7569
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: His6 MBP TEV HDAC8_374
• Alt name: histone deacetylase 8
• Species: H. sapiens (human)
• Insert Size (bp): 1104
• Mutation: Residues Met1–Pro7 and H375-V377 have been deleted relative to wild-type histone deacetylase 8
• Entrez Gene: HDAC8 (a.k.a. CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS)
• Promoter: T7
• Tags / Fusion Proteins:
  • hexahistidine tag (N terminal on backbone)
  • tobacco etch virus protease cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry