M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1
(Plasmid #122147)

• Purpose: Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.
• Depositing Lab: Clifford Brangwynne
• Publication: Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13. doi: 10.1016/j.cell.2018.10.048.

Backbone

• Vector backbone: pHR
• Backbone size w/o insert (bp): 9000
• Total vector size (bp): 10888
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FTH1
• Species: H. sapiens (human)
• Entrez Gene: FTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, NBIA9, PIG15, PLIF)
• Promoter: SFFV
• Tag / Fusion Protein:
  • NLS-iLID-GFP (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector contains iLID, an engineered LOV2-SsrA protein for light dependent heterodimerization