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Addgene

pIVT/Smarca4
(Plasmid #122146)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122146 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIVT2
  • Backbone manufacturer
    Carmen Williams
  • Backbone size w/o insert (bp) 3059
  • Modifications to backbone
    Mouse Smarca4 inserted between Sal1 and Xba1 in pIVT2.
  • Vector type
    For in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smarca4
  • Alt name
    Brg1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4845
  • Entrez Gene
    Smarca4 (a.k.a. BAF190A, Brg1, HP1-BP72, SNF2beta, SW1/SNF, b2b508.1Clo, b2b692Clo)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Smarca4 is from Addgene plasmid #25855.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVT/Smarca4 was a gift from Carmen Williams (Addgene plasmid # 122146 ; http://n2t.net/addgene:122146 ; RRID:Addgene_122146)

  • For your References section:

    Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse. Miao YL, Gambini A, Zhang Y, Padilla-Banks E, Jefferson WN, Bernhardt ML, Huang W, Li L, Williams CJ. Biol Reprod. 2018 Jan 9. pii: 4794936. doi: 10.1093/biolre/ioy004. 10.1093/biolre/ioy004 PubMed 29325037
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