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pF208wt-luc
(Plasmid #122143)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122143 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.10[luc2]
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4242
  • Modifications to backbone
    Fam208a promoter (wt) inserted in pGL4.10[1luc2] between XhoI and HindIII sites
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fam208a promoter
  • Alt name
    D14Abb1e
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1101
  • Entrez Gene
    Tasor (a.k.a. 4732418E12, 4933409E02Rik, D14Abb1, D14Abb1e, Fam20, Fam208a, MommeD, MommeD6)
  • Promoter Fam208a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer TAGCAAAATAGGCTGTCCC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pF208wt-luc was a gift from Carmen Williams (Addgene plasmid # 122143 ; http://n2t.net/addgene:122143 ; RRID:Addgene_122143)

  • For your References section:

    Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse. Miao YL, Gambini A, Zhang Y, Padilla-Banks E, Jefferson WN, Bernhardt ML, Huang W, Li L, Williams CJ. Biol Reprod. 2018 Jan 9. pii: 4794936. doi: 10.1093/biolre/ioy004. 10.1093/biolre/ioy004 PubMed 29325037
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