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Addgene

pIVT/CamKII gamma CA
(Plasmid #122142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIVT
  • Backbone manufacturer
    Carmen Williams
  • Backbone size w/o insert (bp) 3059
  • Vector type
    For in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    constitutively active CamKII gamma
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    873
  • Mutation
    First 291 amino acid residues of CaMKII gamma
  • Entrez Gene
    Camk2g (a.k.a. Camkg)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pst1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVT/CamKII gamma CA was a gift from Carmen Williams (Addgene plasmid # 122142 ; http://n2t.net/addgene:122142 ; RRID:Addgene_122142)

  • For your References section:

    Calcium influx-mediated signaling is required for complete mouse egg activation. Miao YL, Stein P, Jefferson WN, Padilla-Banks E, Williams CJ. Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4169-74. Epub 2012 Feb 27. 10.1073/pnas.1112333109 PubMed 22371584
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