Skip to main content
Addgene

pIVT/Acrosin-GFP-GPI
(Plasmid #122141)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122141 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pIVT2
  • Backbone manufacturer
    Carmen Williams
  • Backbone size w/o insert (bp) 3059
  • Vector type
    For in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    acrosin-GFP-GPI
  • Species
    Synthetic
  • Insert Size (bp)
    918
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    acrosin-GFP-GPI source: PMID: 16604528

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVT/Acrosin-GFP-GPI was a gift from Carmen Williams (Addgene plasmid # 122141 ; http://n2t.net/addgene:122141 ; RRID:Addgene_122141)

  • For your References section:

    Oviductal estrogen receptor alpha signaling prevents protease-mediated embryo death. Winuthayanon W, Bernhardt ML, Padilla-Banks E, Myers PH, Edin ML, Lih FB, Hewitt SC, Korach KS, Williams CJ. Elife. 2015 Dec 1;4:e10453. doi: 10.7554/eLife.10453. 10.7554/eLife.10453 PubMed 26623518
Related items: