pIVT2
(Plasmid
#122140)
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Purpose(Empty Backbone) For making cRNA appropriate for microinjection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122140 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIVT
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Backbone manufacturerCarmen Williams
- Backbone size (bp) 3059
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Modifications to backboneATG of SphI site in the original pIVT was changed to GTG. MCS: Pst1, Sal1, Xba1, BamHI, SmaI, Acc651, KpnI, SacI
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Vector typeFor in vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIVT2 was a gift from Carmen Williams (Addgene plasmid # 122140 ; http://n2t.net/addgene:122140 ; RRID:Addgene_122140) -
For your References section:
Oviductal estrogen receptor alpha signaling prevents protease-mediated embryo death. Winuthayanon W, Bernhardt ML, Padilla-Banks E, Myers PH, Edin ML, Lih FB, Hewitt SC, Korach KS, Williams CJ. Elife. 2015 Dec 1;4:e10453. doi: 10.7554/eLife.10453. 10.7554/eLife.10453 PubMed 26623518