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Addgene

pROP-PP-FBFP
(Plasmid #122137)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122137 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC101
  • Vector type
    Bacterial Expression
  • Selectable markers
    Spectinomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FBFP
  • Promoter J23101

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ccctttttctttaaaaccgaaaaga
  • 3′ sequencing primer cccaatgataaccccCTAGGTCTAGGGCGGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pROP-PP-FBFP was a gift from Srivatsan Raman (Addgene plasmid # 122137 ; http://n2t.net/addgene:122137 ; RRID:Addgene_122137)

  • For your References section:

    A Regulatory NADH/NAD+ Redox Biosensor for Bacteria. Liu Y, Landick R, Raman S. ACS Synth Biol. 2019 Feb 15;8(2):264-273. doi: 10.1021/acssynbio.8b00485. Epub 2019 Jan 23. 10.1021/acssynbio.8b00485 PubMed 30633862
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