pTol2-elavl3_NES-rsCaMPARI-mRuby3
(Plasmid
#122129)
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PurposePan-neuronal expression of rsCaMPARI in zebrafish, nucleus excluded
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122129 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTol2-elavl3
- Backbone size w/o insert (bp) 12542
- Total vector size (bp) 14573
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namersCaMPARI
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SpeciesSynthetic
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Insert Size (bp)2031
- Promoter elavl3
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Tags
/ Fusion Proteins
- NES-His (N terminal on insert)
- mRuby3 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTol2-elavl3_NES-rsCaMPARI-mRuby3 was a gift from Eric Schreiter (Addgene plasmid # 122129 ; http://n2t.net/addgene:122129 ; RRID:Addgene_122129) -
For your References section:
Erasable labeling of neuronal activity using a reversible calcium marker. Sha F, Abdelfattah AS, Patel R, Schreiter ER. Elife. 2020 Sep 15;9. pii: 57249. doi: 10.7554/eLife.57249. 10.7554/eLife.57249 PubMed 32931424
