Foxf1 HA pShuttle
(Plasmid
#122120)
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PurposeExpression of Foxf1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 122120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepShuttle
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFoxf1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1062
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Entrez GeneFoxf1 (a.k.a. FREAC1, Foxf1a, Freac-1, HFH-8, Hfh8)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Foxf1 HA pShuttle was a gift from Paul Herring (Addgene plasmid # 122120 ; http://n2t.net/addgene:122120 ; RRID:Addgene_122120) -
For your References section:
The transcription factor Foxf1 binds to serum response factor and myocardin to regulate gene transcription in visceral smooth muscle cells. Hoggatt AM, Kim JR, Ustiyan V, Ren X, Kalin TV, Kalinichenko VV, Herring BP. J Biol Chem. 2013 Oct 4;288(40):28477-87. doi: 10.1074/jbc.M113.478974. Epub 2013 Aug 14. 10.1074/jbc.M113.478974 PubMed 23946491