Brm BDmut-Flag pShuttle
(Plasmid #122108)

• Purpose: Expression of full length Brm containing point mutations in its bromodomain in mammalian cells
• Depositing Lab: Paul Herring
• Publication: Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;29(6):921-8. doi: 10.1161/ATVBAHA.109.187229. Epub 2009 Apr 2.

Backbone

• Vector backbone: pShuttle
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Brm
• Alt name: SMARCA2
• Species: H. sapiens (human)
• Insert Size (bp): 5000
• Entrez Gene: SMARCA2 (a.k.a. BAF190, BIS, BRM, NCBRS, SNF2, SNF2L2, SNF2LA, SWI2, Sth1p, hBRM, hSNF2a)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • Brm BDmut pShuttle seq

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry