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Addgene

pRC/RSV-rat Agt
(Plasmid #122104)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122104 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRC/RSV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    angiotensinogen
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1400
  • Entrez Gene
    Agt (a.k.a. ANRT, Ang, AngII, PAT)
  • Promoter RSV promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRC/RSV-rat Agt was a gift from John Chan (Addgene plasmid # 122104 ; http://n2t.net/addgene:122104 ; RRID:Addgene_122104)
  • For your References section:

    Essential role(s) of the intrarenal renin-angiotensin system in transforming growth factor-beta1 gene expression and induction of hypertrophy of rat kidney proximal tubular cells in high glucose. Zhang SL, To C, Chen X, Filep JG, Tang SS, Ingelfinger JR, Chan JS. J Am Soc Nephrol. 2002 Feb;13(2):302-12. PubMed 11805157