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Addgene

pS448∙CsR
(Plasmid #122096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA448
  • Backbone manufacturer
    de Lorenzo´s lab
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • Species
    Streptococcus pyogenes
  • GenBank ID
    GI:81533697
  • Promoter Pm

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer TCCAGCCTTGCAAGAAGC
  • 3′ sequencing primer GCGGCAACCGAGCGTTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    sgRNA
  • Alt name
    single guide RNA
  • Alt name
    crRNA - tracrRNA fusion construct
  • Promoter Pem7

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pS448∙CsR was a gift from Pablo Ivan Nikel (Addgene plasmid # 122096 ; http://n2t.net/addgene:122096 ; RRID:Addgene_122096)

  • For your References section:

    Accelerated genome engineering of Pseudomonas putida by I-SceI-mediated recombination and CRISPR-Cas9 counterselection. Wirth NT, Kozaeva E, Nikel PI. Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13396. 10.1111/1751-7915.13396 PubMed 30861315
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