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Addgene

pSEVA1213S
(Plasmid #122095)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122095 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA121
  • Backbone manufacturer
    de Lorenzo´s lab
  • Backbone size w/o insert (bp) 3224
  • Total vector size (bp) 4673

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    I-SceI restriction endonuclease
  • Alt name
    Meganuclease I-SceI
  • Alt name
    homing endonuclease I-SceI
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    886

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVA1213S was a gift from Pablo Ivan Nikel (Addgene plasmid # 122095 ; http://n2t.net/addgene:122095 ; RRID:Addgene_122095)

  • For your References section:

    Accelerated genome engineering of Pseudomonas putida by I-SceI-mediated recombination and CRISPR-Cas9 counterselection. Wirth NT, Kozaeva E, Nikel PI. Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13396. 10.1111/1751-7915.13396 PubMed 30861315
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