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NmCas9_sgRNA_expression_in_pBluescript
(Plasmid #122091)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122091 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS
  • Backbone size w/o insert (bp) 4314
  • Total vector size (bp) 4407
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NmCas9 tracrRNA
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    93
  • Entrez Gene
    NEWENTRY
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BfuAI (destroyed during cloning)
  • 3′ cloning site BfuAI (destroyed during cloning)
  • 5′ sequencing primer T3 Promoter
  • 3′ sequencing primer T7 Promoter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To design oligos for cloning: 5'-ACCG-Spacer_Sequence-3' and 5'-AAAC-Rev_Comp_Spacer-3'
For sgRNA cloning: Digest with BfuAI and ligate in phosphorylated and annealed oligos

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NmCas9_sgRNA_expression_in_pBluescript was a gift from Scot Wolfe (Addgene plasmid # 122091 ; http://n2t.net/addgene:122091 ; RRID:Addgene_122091)

  • For your References section:

    Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing. Bolukbasi MF, Liu P, Luk K, Kwok SF, Gupta A, Amrani N, Sontheimer EJ, Zhu LJ, Wolfe SA. Nat Commun. 2018 Nov 19;9(1):4856. doi: 10.1038/s41467-018-07310-x. 10.1038/s41467-018-07310-x PubMed 30451839
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