SpCas9_sgRNA_expression_in_pBluescript Citations (2)

Originally described in: Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing.
Bolukbasi MF, Liu P, Luk K, Kwok SF, Gupta A, Amrani N, Sontheimer EJ, Zhu LJ, Wolfe SA Nat Commun. 2018 Nov 19;9(1):4856. doi: 10.1038/s41467-018-07310-x. PubMed Journal

Articles Citing SpCas9_sgRNA_expression_in_pBluescript

Articles
Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag. Liang SQ, Liu P, Ponnienselvan K, Suresh S, Chen Z, Kramme C, Chatterjee P, Zhu LJ, Sontheimer EJ, Xue W, Wolfe SA. Nat Methods. 2023 Jun;20(6):898-907. doi: 10.1038/s41592-023-01859-2. Epub 2023 May 8. PubMed Associated Plasmids pET-21a-PE2-His pegRNA_HEK4_20-7 pegRNA_HEK4_G-T pegRNA_HEK4_1bp-deletion pegRNA_HEK4_1bp-insertion
Reducing the inherent auto-inhibitory interaction within the pegRNA enhances prime editing efficiency. Ponnienselvan K, Liu P, Nyalile T, Oikemus S, Maitland SA, Lawson ND, Luban J, Wolfe SA. Nucleic Acids Res. 2023 May 29:gkad456. doi: 10.1093/nar/gkad456. PubMed Associated Plasmids PE-Max-pet21A PEmax-mRNA

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