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Addgene

pGNW4
(Plasmid #122088)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122088 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEMG
  • Backbone manufacturer
    Esteban Martínez-García and Víctor de Lorenzo
  • Backbone size (bp) 3168
  • Modifications to backbone
    introduction of a constitutively expressed monomeric superfolder GFP and exchange of the antibiotic resistance to streptomycin.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5α λpir
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
  • 3′ sequencing primer ATGACCATGATTACGCCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGNW4 was a gift from Pablo Ivan Nikel (Addgene plasmid # 122088 ; http://n2t.net/addgene:122088 ; RRID:Addgene_122088)

  • For your References section:

    Accelerated genome engineering of Pseudomonas putida by I-SceI-mediated recombination and CRISPR-Cas9 counterselection. Wirth NT, Kozaeva E, Nikel PI. Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13396. 10.1111/1751-7915.13396 PubMed 30861315
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