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Purpose(Empty Backbone) suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122086 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEMG
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Backbone manufacturerEsteban Martínez-García and Víctor de Lorenzo
- Backbone size (bp) 3168
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Modifications to backboneIntroduction of a constitutively expressed monomeric superfolder GFP
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5α λpir
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Copy numberLow Copy
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer TGTAAAACGACGGCCAGT
- 3′ sequencing primer ATGACCATGATTACGCCGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGNW2 was a gift from Pablo Ivan Nikel (Addgene plasmid # 122086 ; http://n2t.net/addgene:122086 ; RRID:Addgene_122086) -
For your References section:
Accelerated genome engineering of Pseudomonas putida by I-SceI-mediated recombination and CRISPR-Cas9 counterselection. Wirth NT, Kozaeva E, Nikel PI. Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13396. 10.1111/1751-7915.13396 PubMed 30861315
Map uploaded by the depositor.
