pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C
(Plasmid #122085)

• Purpose: Express M.EcoGII fused to CENPC with destabilization domain - inducible
• Depositing Lab: Laure Crabbe
• Publication: Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5. doi: 10.1016/j.celrep.2018.11.027.

Backbone

• Vector backbone: pRetroX-pTuner
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5875
• Total vector size (bp): 10249
• Vector type: Mammalian Expression, Bacterial Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DD-linker-M.EcoGII-V5-CENPC
• Species: H. sapiens (human); E.coli
• Insert Size (bp): 4374
• Mutation: L341F, R930L (see comments below)
• Entrez Gene: CENPC (a.k.a. CENP-C, CENPC1, MIF2, hcp-4)
• Tag / Fusion Protein:
  • V5 tag on Nter of CENPC (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTGACTATATCTCCAG
• 3′ sequencing primer: GTCAAGAAGACAGGGC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

L341F and R930L mutations do not affect plasmid function. They are common in other isoforms or primate transcripts respectively.