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Addgene

pCuHA-SpAtg101(414)
(Plasmid #122081)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS414
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SpATG101
  • Species
    S. pombe (fission yeast)
  • Tag / Fusion Protein
    • 3HA

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCuHA-SpAtg101(414) was a gift from Daniel Klionsky (Addgene plasmid # 122081 ; http://n2t.net/addgene:122081 ; RRID:Addgene_122081)

  • For your References section:

    Conserved and unique features of the fission yeast core Atg1 complex. Nanji T, Liu X, Chew LH, Li FK, Biswas M, Yu ZQ, Lu S, Dong MQ, Du LL, Klionsky DJ, Yip CK. Autophagy. 2017;13(12):2018-2027. doi: 10.1080/15548627.2017.1382782. Epub 2017 Nov 23. 10.1080/15548627.2017.1382782 PubMed 28976798
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