pCuGFP-SpAtg17(416)
(Plasmid
#122080)
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PurposeExpressing S. pombe GFP-Atg17
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122080 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS416
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSpATG17
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SpeciesS. pombe (fission yeast)
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Entrez Geneatg17 (a.k.a. SPAC10F6.11c)
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Tag
/ Fusion Protein
- GFP
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCuGFP-SpAtg17(416) was a gift from Daniel Klionsky (Addgene plasmid # 122080 ; http://n2t.net/addgene:122080 ; RRID:Addgene_122080) -
For your References section:
Conserved and unique features of the fission yeast core Atg1 complex. Nanji T, Liu X, Chew LH, Li FK, Biswas M, Yu ZQ, Lu S, Dong MQ, Du LL, Klionsky DJ, Yip CK. Autophagy. 2017;13(12):2018-2027. doi: 10.1080/15548627.2017.1382782. Epub 2017 Nov 23. 10.1080/15548627.2017.1382782 PubMed 28976798