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Addgene

pCuHA-ATG13[3SD](426)
(Plasmid #122077)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS426
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATG13
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Serine 646 to aspartic acid; Serine 649 to aspartic acid; Serine 652 to aspartic acid
  • Tag / Fusion Protein
    • 3HA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor notes that the D308G and H545Y substitutions found in ATG13, are not of concern.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCuHA-ATG13[3SD](426) was a gift from Daniel Klionsky (Addgene plasmid # 122077 ; http://n2t.net/addgene:122077 ; RRID:Addgene_122077)

  • For your References section:

    Molecular interactions of the Saccharomyces cerevisiae Atg1 complex provide insights into assembly and regulatory mechanisms. Chew LH, Lu S, Liu X, Li FK, Yu AY, Klionsky DJ, Dong MQ, Yip CK. Autophagy. 2015;11(6):891-905. doi: 10.1080/15548627.2015.1040972. 10.1080/15548627.2015.1040972 PubMed 25998554
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