pGFP-ATG13[3SD](315)
(Plasmid
#122074)
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PurposeExpressing GFP-Atg13[3SD]
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 122074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS315
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameATG13
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SpeciesS. cerevisiae (budding yeast)
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MutationSerine 646 to aspartic acid; Serine 649 to aspartic acid; Serine 652 to aspartic acid;
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Tag
/ Fusion Protein
- GFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGFP-ATG13[3SD](315) was a gift from Daniel Klionsky (Addgene plasmid # 122074 ; http://n2t.net/addgene:122074 ; RRID:Addgene_122074) -
For your References section:
Molecular interactions of the Saccharomyces cerevisiae Atg1 complex provide insights into assembly and regulatory mechanisms. Chew LH, Lu S, Liu X, Li FK, Yu AY, Klionsky DJ, Dong MQ, Yip CK. Autophagy. 2015;11(6):891-905. doi: 10.1080/15548627.2015.1040972. 10.1080/15548627.2015.1040972 PubMed 25998554