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pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)
(Plasmid #122067)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122067 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS405
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dhh1-PA
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Aspartic acid 195 to alanine; Glutamic acid 196 to alanine; Serine 226 to alanine; Threonine 228 to alanine
  • Entrez Gene
    DHH1 (a.k.a. YDL160C)
  • Tag / Fusion Protein
    • PA

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405) was a gift from Daniel Klionsky (Addgene plasmid # 122067 ; http://n2t.net/addgene:122067 ; RRID:Addgene_122067)

  • For your References section:

    Dhh1 promotes autophagy-related protein translation during nitrogen starvation. Liu X, Yao Z, Jin M, Namkoong S, Yin Z, Lee JH, Klionsky DJ. PLoS Biol. 2019 Apr 11;17(4):e3000219. doi: 10.1371/journal.pbio.3000219. eCollection 2019 Apr. 10.1371/journal.pbio.3000219 PubMed 30973873
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