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Addgene

pGAL-GFP kan
(Plasmid #122060)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122060 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYES2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5900
  • Total vector size (bp) 8600
  • Modifications to backbone
    kanamycin resistance gene put into ampicillin ORF
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    green fluorescent protein
  • Species
    aequorea victoria
  • Insert Size (bp)
    1000
  • GenBank ID
    M62653
  • Promoter GAL1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Gal1 promoter
  • 3′ sequencing primer cyc1 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGAL-GFP kan was a gift from Daniel Reines (Addgene plasmid # 122060 ; http://n2t.net/addgene:122060 ; RRID:Addgene_122060)

  • For your References section:

    A fluorescent assay for the genetic dissection of the RNA polymerase II termination machinery. Reines D. Methods. 2019 Jan 4. pii: S1046-2023(18)30292-5. doi: 10.1016/j.ymeth.2018.12.011. 10.1016/j.ymeth.2018.12.011 PubMed 30616008
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