pJ3H-Mst2 K56R
(Plasmid #12206)

• Depositing Lab: Jonathan Chernoff
• Publication: Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-53.

Backbone

• Vector backbone: pJ3H
• Backbone size w/o insert (bp): 3500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mst2
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: Catalytically inactive: K56R
• Entrez Gene: STK3 (a.k.a. KRS1, MST2)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SV40pro-F

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's QC finds additional E303D and T342M substitutions which are not known to affect function