CYFIP1.mCherry
(Plasmid
#122051)
-
PurposeExpresses human CYFIP1 in mammalian cells.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122051 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepmcherry-N1
-
Backbone manufacturerClontech
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCYFIP1
-
Alt nameSRA1
-
SpeciesH. sapiens (human)
-
Entrez GeneCYFIP1 (a.k.a. P140SRA-1, SHYC, SRA-1, SRA1)
- Promoter CMV
-
Tag
/ Fusion Protein
- mCherry (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer mCherry-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
CYFIP1.mCherry was a gift from Josef Kittler (Addgene plasmid # 122051 ; http://n2t.net/addgene:122051 ; RRID:Addgene_122051) -
For your References section:
The autism and schizophrenia associated gene CYFIP1 is critical for the maintenance of dendritic complexity and the stabilization of mature spines. Pathania M, Davenport EC, Muir J, Sheehan DF, Lopez-Domenech G, Kittler JT. Transl Psychiatry. 2014 Mar 25;4:e374. doi: 10.1038/tp.2014.16. 10.1038/tp.2014.16 PubMed 24667445
