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Addgene

pJ3M-Mst1 K59R
(Plasmid #12204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ3M
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mst1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    Catalytically inactive: K59R
  • Entrez Gene
    STK4 (a.k.a. KRS2, MST1, YSK3)
  • Entrez Gene
    MST1 (a.k.a. D3F15S2, DNF15S2, HGFL, MSP, NF15S2)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SV40pro-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ3M-Mst1 K59R was a gift from Jonathan Chernoff (Addgene plasmid # 12204 ; http://n2t.net/addgene:12204 ; RRID:Addgene_12204)

  • For your References section:

    The Ste20-like protein kinase, Mst1, dimerizes and contains an inhibitory domain. Creasy CL, Ambrose DM, Chernoff J. J Biol Chem. 1996 Aug 30. 271(35):21049-53. 10.1074/jbc.271.35.21049 PubMed 8702870
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