zCD2
(Plasmid #122031)

• Purpose: Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.
• Depositing Lab: David Christianson
• Publication: Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi: 10.1038/nchembio.2134. Epub 2016 Jul 25.

Backbone

• Vector backbone: pET
• Backbone manufacturer: Scott Gradia
• Backbone size w/o insert (bp): 6465
• Total vector size (bp): 7542
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Zebrafish HDAC6 Catalytic Domain 2
• Alt name: zCD2
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1077
• Promoter: T7
• Tag / Fusion Protein:
  • His6-MBP tag with cleavable TEV site (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original cloning was done by Dr. Yang Hai, a previous Christianson Lab member.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The cloning strategy is outlined in:

Hai, Y.; Christianson, D.W. (2016). Histone deacetylase 6 structure and molecular basis of catalysis and inhibition. Nat Chem Biol. 12(9), 741-7.