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pSEVA228-pro4IUPi
(Plasmid #122018)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA228
  • Backbone manufacturer
    Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B., Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D.
  • Backbone size w/o insert (bp) 3465
  • Total vector size (bp) 7088
  • Modifications to backbone
    Removed xylS gene
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Choline kinase
  • Alt name
    ScCK
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1749
  • Mutation
    Codon optimized for E. coli
  • GenBank ID
    NC_001144.5
  • Entrez Gene
    CKI1 (a.k.a. YLR133W)
  • Promoter pro4

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Isopentenyl monophosphate kinase
  • Alt name
    AtIPK
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    999
  • Mutation
    Codon optimized for E. coli
  • GenBank ID
    NP_173986.2
  • Entrez Gene
    AT1G26640 (a.k.a. AT1G26640, T24P13.2, T24P13_2)
  • Promoter pro4

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    isopentenyl-diphosphate Delta-isomerase
  • Alt name
    idi
  • Species
    Escherichia coli str. K-12 substr. MG1655
  • Insert Size (bp)
    549
  • GenBank ID
    NC_000913.3
  • Promoter pro4

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVA228-pro4IUPi was a gift from Gregory Stephanopoulos (Addgene plasmid # 122018 ; http://n2t.net/addgene:122018 ; RRID:Addgene_122018)

  • For your References section:

    Two-step pathway for isoprenoid synthesis. Chatzivasileiou AO, Ward V, Edgar SM, Stephanopoulos G. Proc Natl Acad Sci U S A. 2018 Dec 24. pii: 1812935116. doi: 10.1073/pnas.1812935116. 10.1073/pnas.1812935116 PubMed 30584096
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