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Addgene

pNIA-CEN-FLAG-LANS1-Set2
(Plasmid #122002)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122002 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIA-CEN
  • Backbone size w/o insert (bp) 8138
  • Total vector size (bp) 10808
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    histone methyltransferase SET2
  • Alt name
    FLAG-LANS-Set2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2670
  • Mutation
    Set2 point mutations K538G, K539S, R549G, K550S, K551G
  • Entrez Gene
    SET2 (a.k.a. YJL168C, EZL1, KMT3)
  • Promoter ADH1
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTCATTGTTCTCGTTCCCTTTCTTCCTTG
  • 3′ sequencing primer GGGACCTAGACTTCAGGTTGTCTAACTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: the Addgene ID for this plasmid is incorrectly listed in the publication as 122003. Addgene plasmid 122003 is mVenus-Flag-LANS-Set2 and Addgene plasmid 122002 is Flag-LANS-Set2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIA-CEN-FLAG-LANS1-Set2 was a gift from Brian Kuhlman (Addgene plasmid # 122002 ; http://n2t.net/addgene:122002 ; RRID:Addgene_122002)

  • For your References section:

    An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation. Lerner AM, Hepperla AJ, Keele GR, Meriesh HA, Yumerefendi H, Restrepo D, Zimmerman S, Bear JE, Kuhlman B, Davis IJ, Strahl BD. Genome Res. 2020 Nov;30(11):1605-1617. doi: 10.1101/gr.264283.120. Epub 2020 Oct 5. 10.1101/gr.264283.120 PubMed 33020206
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