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Purpose(Empty Backbone) A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a and pWH1266
- Backbone size (bp) 12095
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Modifications to backbonepBECAb-apr was constructed by combining colE1_origin (form pET28a vector) and WH1266_origin (from pWH1266 vector).
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsSelectable Marker: 100 μg/mL apramycin in both E.coli DH5alpha and Acinetobacter baumannii
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBECAb-apr was a gift from Quanjiang Ji (Addgene plasmid # 122001 ; http://n2t.net/addgene:122001 ; RRID:Addgene_122001) -
For your References section:
A Highly Efficient CRISPR-Cas9-Based Genome Engineering Platform in Acinetobacter baumannii to Understand the H2O2-Sensing Mechanism of OxyR. Wang Y, Wang Z, Chen Y, Hua X, Yu Y, Ji Q. Cell Chem Biol. 2019 Sep 17. pii: S2451-9456(19)30277-6. doi: 10.1016/j.chembiol.2019.09.003. 10.1016/j.chembiol.2019.09.003 PubMed 31548010
