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PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumannii
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122000 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57 and pWH1266
- Backbone size w/o insert (bp) 6049
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Modifications to backbonepSGAb-spe was constructed by combining two different replication regions: pMB1_origin (from pUC57 vector) and WH1266_origin (from pWH1266 vector)
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsSelectable Marker: 50 μg/mL spectinomycin in E.coli DH5alpha, 100 μg/mL in Acinetobacter baumannii
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenone
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gRNA/shRNA sequenceunknown
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSGAb-spe was a gift from Quanjiang Ji (Addgene plasmid # 122000 ; http://n2t.net/addgene:122000 ; RRID:Addgene_122000) -
For your References section:
A Highly Efficient CRISPR-Cas9-Based Genome Engineering Platform in Acinetobacter baumannii to Understand the H2O2-Sensing Mechanism of OxyR. Wang Y, Wang Z, Chen Y, Hua X, Yu Y, Ji Q. Cell Chem Biol. 2019 Sep 17. pii: S2451-9456(19)30277-6. doi: 10.1016/j.chembiol.2019.09.003. 10.1016/j.chembiol.2019.09.003 PubMed 31548010
