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p2HGAL/GR/CYC
(Plasmid #1220)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1220 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS423
  • Backbone manufacturer
    atcc
  • Backbone size w/o insert (bp) 9200
  • Vector type
    Yeast Expression ; 2micron

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GR
  • Alt name
    glucocorticoid receptor
  • Alt name
    Nr3c1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2400
  • Mutation
    pRS423 with GAL promoter and CYC terminator, 2micron, select for using HIS3
  • Entrez Gene
    Nr3c1 (a.k.a. GR, Gcr, Grl)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    see comments

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from plasmids from the following reference: Picard D, Khurseed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto
KR. Reduced levels of hsp90 compromise steroid receptor action in
vivo. Nature (1990), 348 (6297): 166-8.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2HGAL/GR/CYC was a gift from Susan Lindquist (Addgene plasmid # 1220 ; http://n2t.net/addgene:1220 ; RRID:Addgene_1220)

  • For your References section:

    Mutational analysis of Hsp90 function: interactions with a steroid receptor and a protein kinase. Nathan DF, Lindquist S. Mol Cell Biol 1995 Jul;15(7):3917-25. PubMed 7791797
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