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Addgene

pSGAb-km
(Plasmid #121999)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121999 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57 and pWH1266
  • Backbone size w/o insert (bp) 6073
  • Modifications to backbone
    pSGAb-km was constructed by combining two different replication regions: pMB1_origin (from pUC57 vector) and WH1266_origin (from pWH1266 vector).
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Selectable Marker: 50 μg/mL kanamycin in both E.coli DH5alpha and Acinetobacter baumannii
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    none
  • gRNA/shRNA sequence
    unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSGAb-km was a gift from Quanjiang Ji (Addgene plasmid # 121999 ; http://n2t.net/addgene:121999 ; RRID:Addgene_121999)

  • For your References section:

    A Highly Efficient CRISPR-Cas9-Based Genome Engineering Platform in Acinetobacter baumannii to Understand the H2O2-Sensing Mechanism of OxyR. Wang Y, Wang Z, Chen Y, Hua X, Yu Y, Ji Q. Cell Chem Biol. 2019 Sep 17. pii: S2451-9456(19)30277-6. doi: 10.1016/j.chembiol.2019.09.003. 10.1016/j.chembiol.2019.09.003 PubMed 31548010
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