pMYs-MFN2-IRES-mitoRFP
(Plasmid
#121997)
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PurposeExpression of MFN2 and mitoRFP in mammalial cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121997 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMYs
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Backbone manufacturerCell Biolabs
- Backbone size w/o insert (bp) 4800
- Total vector size (bp) 8531
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMFN2-IRES-mitoRFP
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SpeciesH. sapiens (human)
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Insert Size (bp)3739
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Mutationsilent Q386Q Q387Q V388V Y389Y C390C E391E E392E
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Entrez GeneMFN2 (a.k.a. CMT2A, CMT2A2, CMT2A2A, CMT2A2B, CPRP1, HMSN6A, HSG, MARF, MSL)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMYs-MFN2-IRES-mitoRFP was a gift from Qing Deng (Addgene plasmid # 121997 ; http://n2t.net/addgene:121997 ; RRID:Addgene_121997) -
For your References section:
Mitofusin 2 regulates neutrophil adhesive migration and the actin cytoskeleton. Zhou W, Hsu AY, Wang Y, Syahirah R, Wang T, Jeffries J, Wang X, Mohammad H, Seleem MN, Umulis D, Deng Q. J Cell Sci. 2020 Sep 4;133(17). pii: jcs.248880. doi: 10.1242/jcs.248880. 10.1242/jcs.248880 PubMed 32788232