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p3E-U6a-U6c-opa1 guide
(Plasmid #121995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3E
  • Backbone manufacturer
    Tol2 kit
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4061
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    opa1 gRNA
  • gRNA/shRNA sequence
    GTAGTTGGGGACCAGAGTG, GGCAGCTGAGCAGTGAGG
  • Species
    D. rerio (zebrafish)
  • GenBank ID
    ENSDARG00000070801

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3E-U6a-U6c-opa1 guide was a gift from Qing Deng (Addgene plasmid # 121995 ; http://n2t.net/addgene:121995 ; RRID:Addgene_121995)

  • For your References section:

    Mitofusin 2 regulates neutrophil adhesive migration and the actin cytoskeleton. Zhou W, Hsu AY, Wang Y, Syahirah R, Wang T, Jeffries J, Wang X, Mohammad H, Seleem MN, Umulis D, Deng Q. J Cell Sci. 2020 Sep 4;133(17). pii: jcs.248880. doi: 10.1242/jcs.248880. 10.1242/jcs.248880 PubMed 32788232
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