pHBS941 TDP43 RRM-GFP VLIMFYW-S
(Plasmid
#121992)
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PurposeTo test the effect of sequence on TDP43 RRM-GFP droplet properties
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2-DEST
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTARDBP mutant
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SpeciesH. sapiens (human)
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MutationAll hydrophobic residues in IDR1&IDR2 mutated to serine
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Entrez GeneTARDBP (a.k.a. ALS10, TDP-43)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Category 1: Compositional TDP43 CTD mutants
This plasmid is an updated version of Addgene plasmid 107798. Plasmid has two more hydrophobic residues converted to serine.
Please visit https://www.biorxiv.org/content/10.1101/548339v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHBS941 TDP43 RRM-GFP VLIMFYW-S was a gift from Rajat Rohatgi (Addgene plasmid # 121992 ; http://n2t.net/addgene:121992 ; RRID:Addgene_121992) -
For your References section:
Phase separation-deficient TDP43 remains functional in splicing. Schmidt HB, Barreau A, Rohatgi R. Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2. 10.1038/s41467-019-12740-2 PubMed 31653829