pDestTol2-ubi-ASAP1
(Plasmid
#121987)
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Purposeubiqutously express ASAP1 voltage biosensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121987 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDestTol2pA2
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Vector typeTol2 transposon destination vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameASAP1
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SpeciesSynthetic
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Insert Size (bp)1284
- Promoter zebrafish ubiquitin promoter
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDestTol2-ubi-ASAP1 was a gift from GuangJun Zhang (Addgene plasmid # 121987 ; http://n2t.net/addgene:121987 ; RRID:Addgene_121987) -
For your References section:
Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors. Silic MR, Zhang G. J Vis Exp. 2018 Apr 25;(134). doi: 10.3791/57330. 10.3791/57330 PubMed 29757272